Composite

Part:BBa_M31975:Design

Designed by: Tiffany Guo   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-28)


M13 refactoring


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2562
    Illegal EcoRI site found at 2607
    Illegal XbaI site found at 2656
    Illegal SpeI site found at 1710
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2562
    Illegal EcoRI site found at 2607
    Illegal NheI site found at 2312
    Illegal SpeI site found at 1710
    Illegal NotI site found at 2678
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2562
    Illegal EcoRI site found at 2607
    Illegal BglII site found at 2259
    Illegal BamHI site found at 3327
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2562
    Illegal EcoRI site found at 2607
    Illegal XbaI site found at 2656
    Illegal SpeI site found at 1710
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2562
    Illegal EcoRI site found at 2607
    Illegal XbaI site found at 2656
    Illegal SpeI site found at 1710
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1) eliminate rbs and promoters within genes: took into consideration codon changes that would not change amino acid sequences but would decrease the strength of the rbs or promoter. 2) add restriction sites between each part: tried to keep sticky ends as consistent as possible (i.e. after every promoter, the restriction site sticky end is 3'ctag), however, there aren't enough zero cutting isoschizomers.


Source

parts from M13K07 genome

References